Practical - Merten Lab
BIO-631 / 1 crédit
Enseignant: Merten Christoph
Langue: Anglais
Remark: Open to max. 4 students. 3-day Block course, every year in January. To register, contact EDMS administration.
Frequency
Every year
Summary
Learn how to design & perform microfluidic droplet experiments for single-cell encapsulation. Through hands-on work, gain experience with chip operation, droplet generation & fluorescent signal acquisition. Introduction to single-cell genomics workflows & their integration with microfluidic technol.
Content
This three-day course offers an immersive introduction to microfluidic technologies and their applications in single-cell biology. Participants will be exposed to the principles of microfabrication processes and chip design features. They will perform hands-on droplet generation experiments and single-cell encapsulation, tuning cell occupancy in droplets and evaluate how flow parameters affect droplet size and generation rate. They will also learn the principles of fluorescence signal acquisition on our custom-made detection technology. Finally, students will also familiarize with 10x Genomics single-cell library preparation and next-generation sequencing (NGS) workflows. By the end of the course, students will have a solid understanding of how microfluidics can be leveraged for high-throughput single-cell analyses and will be prepared to integrate these methods into their own research.
Note
Please note that you are not allowed to register in your own lab!
Keywords
Microfluidics, Droplet Generation, Single-Cell Analysis, Next-Generation Sequencing, 10x Genomics
Learning Outcomes
By the end of the course, the student must be able to:
- Explain the principles of microfluidic chip fabrication and droplet-based methodologies for single-cell applications.
- Operate microfluidic chips to generate droplets and control encapsulation parameters.
- Understand and design phenotypic single-cell assays.
- Summarize the main steps of 10x Genomics gene-expression technologies and single-cell library preparation for next-generation sequencing.
Assessment methods
Oral presentation
Dans les plans d'études
- Nombre de places: 4
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Practical - Merten Lab
- Cours: 4 Heure(s)
- Exercices: 18 Heure(s)
- Type: optionnel