BIO-679 / 1 crédit

Enseignant: Suter David Michael

Langue: Anglais

Remark: 3-day Block course, every year in January. To register, contact EDMS Administration


Frequency

Every year

Summary

Bioluminescence imaging and data analysis Splinkerette PCR (to analyze genomic insertion site of a transgene). The students will obtain theoretical and practical insight into embryonic stem cell biology and the study of gene expression fluctuations in single cells.

Content

The course will start out with a lecture and a discussion on stochastic gene expression and how it impacts cell fate choices in stem cells. The different methods to study gene expression at the single cell level will be discussed, as well as experimental strategies to link gene expression fluctuations to cell fate decisions.

In the practical part of the course the students will learn how to measure gene expression in single embryonic stem cells, to analyze the data and to determine the genomic insertion site of a reporter gene.

Note

Please note that you are not allowed to inscribe in your own group!

Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.

Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!

Keywords

Embryonic stem cells, stochastic gene expression, cell fate choice.

Learning Prerequisites

Recommended courses

Basic molecular biology.

Assessment methods

Oral

Resources

Websites

Dans les plans d'études

  • Nombre de places: 3
  • Forme de l'examen: Oral (session libre)
  • Matière examinée: Practical - Suter Lab
  • Cours: 6 Heure(s)
  • TP: 18 Heure(s)
  • Type: obligatoire

Semaine de référence

Cours connexes

Résultats de graphsearch.epfl.ch.