Practical - Suter Lab
BIO-679 / 1 credit
Teacher: Suter David Michael
Language: English
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
Bioluminescence imaging and data analysis Splinkerette PCR (to analyze genomic insertion site of a transgene). The students will obtain theoretical and practical insight into embryonic stem cell biology and the study of gene expression fluctuations in single cells.
Content
The course will start out with a lecture and a discussion on stochastic gene expression and how it impacts cell fate choices in stem cells. The different methods to study gene expression at the single cell level will be discussed, as well as experimental strategies to link gene expression fluctuations to cell fate decisions.
In the practical part of the course the students will learn how to measure gene expression in single embryonic stem cells, to analyze the data and to determine the genomic insertion site of a reporter gene.
Note
Please note that you cannot register in your own group Practical!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Keywords
Embryonic stem cells, stochastic gene expression, cell fate choice.
Learning Prerequisites
Recommended courses
Basic molecular biology.
Assessment methods
Oral
In the programs
- Number of places: 3
- Exam form: Oral (session free)
- Subject examined: Practical - Suter Lab
- Lecture: 6 Hour(s)
- Practical work: 18 Hour(s)
- Type: mandatory