Practical - Constam Lab
BIO-611 / 1 crédit
Enseignant: Constam Daniel
Langue: Anglais
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
During development, cell fates are governed by multiple microenvironmental cues and their integration by specific signal transduction pathways. This course focuses on imaging of mechanosensory cilia or of molecules implicated in specific signal transduction events during mammalian embryogenesis.
Content
Analysis of RNA-protein interactions by EMSA assays
- Preparation, handling and characterization of RNA in vitro:
- PCR amplification and purification of a template DNA for in vitro transcription
- In vitro transcription and purification of RNAs
- Spectrophotometric analysis of purified RNAs
- Characterization of recombinant protein-RNA complexes in vitro:
- Electrophoretic Mobility Shift Assay (EMSA) using fluorescently labelled RNA probes
- Determination of binding affinities by calculation of dissociation constant (Kd)
Note
Please note that you are not allowed to inscribe in your own group!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Keywords
Embryogenesis, cancer, proteases, TGFb signaling, primary cilia, imaging
Learning Prerequisites
Recommended courses
Basics of molecular and cell biology.
Assessment methods
Quiz (multiple choice questions)
Dans les plans d'études
- Nombre de places: 2
- Forme de l'examen: Ecrit (session libre)
- Matière examinée: Practical - Constam Lab
- Cours: 7 Heure(s)
- TP: 20 Heure(s)