Practical - Constam Lab
BIO-611 / 1 credit
Teacher: Constam Daniel
Language: English
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
Primary cilia have emerged as an important organelle of cells to sense environmental cues that control embryogenesis and adult renal tubule and liver bile duct homoestasis. This course focuses on functional analysis of molecules implicated in signal transduction of fluid flow-sensing cilia.
Content
Analysis of RNA-protein interactions by EMSA assays
- Preparation, handling and characterization of RNA in vitro:
- PCR amplification and purification of a template DNA for in vitro transcription
- In vitro transcription and purification of RNAs
- Spectrophotometric analysis of purified RNAs
- Characterization of recombinant protein-RNA complexes in vitro:
- Electrophoretic Mobility Shift Assay (EMSA) using fluorescently labelled RNA probes
- Determination of binding affinities by calculation of dissociation constant (Kd)
Note
Please note that you cannot register in your own group Practical!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Keywords
Cancer, polycystic kidney diseases (PKD), TGFb signaling, primary cilia, proteases, imaging
Learning Prerequisites
Recommended courses
Basics of molecular and cell biology.
Assessment methods
Quiz (multiple choice questions)
In the programs
- Number of places: 2
- Exam form: Written (session free)
- Subject examined: Practical - Constam Lab
- Courses: 7 Hour(s)
- TP: 20 Hour(s)
- Type: mandatory