BIO-611 / 1 credit

Teacher: Constam Daniel

Language: English

Remark: 3-day Block course, every year in January. To register, contact EDMS Administration


Frequency

Every year

Summary

Primary cilia have emerged as an important organelle of cells to sense environmental cues that control embryogenesis and adult renal tubule and liver bile duct homoestasis. This course focuses on functional analysis of molecules implicated in signal transduction of fluid flow-sensing cilia.

Content

Analysis of RNA-protein interactions by EMSA assays

  •  Preparation, handling and characterization of RNA in vitro:
    - PCR amplification and purification of a template DNA for in vitro transcription
    - In vitro transcription and purification of RNAs
    - Spectrophotometric analysis of purified RNAs
  • Characterization of recombinant protein-RNA complexes in vitro:
    - Electrophoretic Mobility Shift Assay (EMSA) using fluorescently labelled RNA probes
    - Determination of binding affinities by calculation of dissociation constant (Kd)

Note

Please note that you cannot register in your own group Practical!

Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.

Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!

Keywords

Cancer, polycystic kidney diseases (PKD), TGFb signaling, primary cilia, proteases, imaging

Learning Prerequisites

Recommended courses

Basics of molecular and cell biology.

Assessment methods

Quiz (multiple choice questions)

In the programs

  • Number of places: 2
  • Exam form: Written (session free)
  • Subject examined: Practical - Constam Lab
  • Courses: 7 Hour(s)
  • TP: 20 Hour(s)
  • Type: mandatory

Reference week

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