BIO-410 / 4 crédits

Enseignant(s): Sage Daniel, Seitz Arne

Langue: Anglais


Summary

The course provides a comprehensive overview of methods, algorithms, and computer tools used in bioimage analysis. It exposes fundamental concepts and practical computer solutions to extract quantitative information from multidimensional images, both using engineering methods and deep learning.

Content

To investigate biological processes, bioimage informatics emerges as a growing field on the interface between microscopy, signal-processing, and computer science. The recent microscopes are producing large volumes of high-resolution multidimensional data (up to 5D). Therefore, algorithms and software tools are needed to automatically extract quantitative data from these images.

The course gives the theoretical concepts and practical aspects of the most common image reconstruction and image analysis techniques. It explains how to code algorithms and to deploy software tools to build an automatic analysis workflow using the most common used software, Fiji/ImageJ (Java-based) and Jupyter Notebook (Python-based). The lecture is tailored to the needs of life sciences and driven by biological questions.

Addressed topics include (but not restricted to): presentation of microscopy modalities, digital images, multi-dimensional data (3D, time, multiple channels) manipulation, 3D image-processing algorithms, 5D visualization, image metrics, reconstruction, deconvolution, denoising, stitching, visual feature detection, segmentation, active contours, image analysis workflow, pixel classification, machine learning, deep learning for image analysis, large datasets, tracking of particles, and super-resolution localization microscopy.

The course is composed of lectures, workshops, practices, and a mini-project.

 

A personal laptop is recommended to run (open-source) image analysis software and to develop short scripts.

Keywords

Bioimage, microscopy, image processing, image reconstruction, image analysis, visualization, multidimensional data analysis, machine learning, deep learning

 

Learning Prerequisites

Required courses

  • Baisc knowledge in programming

 

Assessment methods

20% Homework, individual

  • In the first half of the semester: 4 homeworks on computer (2 weeks)

40% Mini-project by groups of 2-3 students

  • In the second half of the semester: Development of an image analysis tool for a real application in biology

40% End-term exam, individual

  • written exam with handwritten notes

Resources

Moodle Link

Dans les plans d'études

  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel
  • Semestre: Printemps
  • Forme de l'examen: Ecrit (session d'été)
  • Matière examinée: Bioimage informatics
  • Cours: 2 Heure(s) hebdo x 14 semaines
  • Projet: 2 Heure(s) hebdo x 14 semaines
  • Type: optionnel

Semaine de référence

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