To investigate biological processes, bioimage informatics emerges as a growing field on the interface between microscopy, signal-processing, and computer science. The recent microscopes are producing large volumes of high-resolution multidimensional data (up to 5D). Therefore, algorithms and software tools are needed to automatically extract quantitative data from these images.

The course gives the theoretical concepts and practical aspects of the most common image reconstruction and image analysis techniques. It explains how to code algorithms and to deploy software tools to build an automatic analysis workflow using the most common used software, Fiji/ImageJ (Java-based) and Jupyter Notebook (Python-based). The lecture is tailored to the needs of life sciences and driven by biological questions.

Addressed topics include (but not restricted to): presentation of microscopy modalities, digital images, multi-dimensional data (3D, time, multiple channels) manipulation, 3D image-processing algorithms, 5D visualization, image metrics, reconstruction, deconvolution, denoising, stitching, visual feature detection, segmentation, active contours, image analysis workflow, pixel classification, machine learning, deep learning for image analysis, large datasets, tracking of particles, and super-resolution localization microscopy.

The course is composed of lectures, workshops, practices, and a mini-project.