Practical - Thomä Lab
BIO-624 / 1 credit
Teacher(s): Invited lecturers (see below), Thomä Nicolas
Language: English
Remark: Open to max. 4 students. 3-day Block course, every year in January. Registration ONLY by EDMS administration.
Frequency
Every year
Summary
Expression and purification of recombinant proteins are key methods in our lab studying protein machines on a structural level and ways in which they can be reprogrammed by small molecules. In the course, we will express and purify proteins and biophysically characterise them.
Content
The following topics are anticipated to be covered in class:
- Expression systems
- Chromatography techniques to purify recombinant proteins
- Biophysical assays
The objective of the course is to equip students with practical, hands-on knowledge on protein expression and purification techniques, particularly in relation to automated systems like the ÄKTA equipment, as well as binding assays.
The curriculum will cover both theoretical concepts and practical skills, such as chromatographic methods, the operation and maintenance of automated ÄKTA systems, the monitoring and verification of purification results using SDS-PAGE, protein concentration and dialysis techniques, as well as cleaning chromatographic columns. We will also characterise binding of proteins using biophysical assays.
Note
Please note that you cannot register in your own group Practical.
Keywords
Protein Expression, Protein Purification, Biophyscial Assays
Learning Outcomes
By the end of the course, the student must be able to:
- conduct and analyze an SDS-PAGE and handle a recombinant protein. Knowledge about different purification techniques and purification tags.
Assessment methods
Oral presentation
In the programs
- Number of places: 4
- Exam form: Oral presentation (session free)
- Subject examined: Practical - Thomä Lab
- Lecture: 6 Hour(s)
- Practical work: 18 Hour(s)
- Type: optional