Practical - Stahlberg Lab
BIO-603(SH) / 1 credit
Teacher: Stahlberg Henning Paul-Julius
Language: English
Remark: 3-day Block course, every year in January. To register, contact EDMS
Frequency
Every year
Summary
Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.
Content
Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.
Note
Please note that you cannot register in your own group Practical!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Keywords
Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction
In the programs
- Number of places: 3
- Exam form: Oral presentation (session free)
- Subject examined: Practical - Stahlberg Lab
- Lecture: 6 Hour(s)
- Exercises: 6 Hour(s)
- Practical work: 16 Hour(s)
- Type: mandatory