Practical - Stahlberg Lab

BIO-603(SH) / 1 credit

Teacher: Stahlberg Henning Paul-Julius

Language: English

Remark: 3-day Block course, every year in January. To register, contact EDMS

Frequency

Every 2 years

Summary

Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.

Content

Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.

Note

Please note that you cannot register in your own group Practical!

Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.

Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!

Keywords

Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction

Resources

Websites

In the programs

  • Number of places: 3
  • Exam form: Oral presentation (session free)
  • Subject examined: Practical - Stahlberg Lab
  • Courses: 6 Hour(s)
  • Exercises: 6 Hour(s)
  • TP: 16 Hour(s)
  • Type: mandatory

Reference week

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