Fiches de cours

Recombinant protein expression in animal cells for appli-cations in medicine and structural biology


Lecturer(s) :

Abriata Luciano Andres
Hacker David
Lau Kelvin Ka Ching
Pojer Florence




Every year


Every year


Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins from animal cells.


Subjects discussed in class are expected to include:

'          Transient gene expression

'          Stable gene expression

'          Cell-free protein synthesis

'          X-ray diffraction

'          Cryogenic electron microscopy

'          Production of therapeutic antibodies

'          Production of transmembrane proteins

'          NMR

'          Biophysical techniques for protein characterization


Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.


Next session for this course : autumn 2020

Starting on Tuesday 15th September and ending on 15th December, taking place every Tuesdays from 3 to 5pm either in room AI 2142 or by Zoom, depending on the COVID sanitary situation by then.

Max. 15 participants. To register, please send an email to


recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure

Learning Prerequisites

Required courses

Basic cell biology

Learning Outcomes

By the end of the course, the student must be able to:

Assessment methods

Oral presentation

In the programs

    • Semester
    • Exam form
       Oral presentation
    • Credits
    • Subject examined
      Recombinant protein expression in animal cells for appli-cations in medicine and structural biology
    • Number of places
    • Lecture
      28 Hour(s)

Reference week

      Exercise, TP
      Project, other


  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German