Recombinant protein expression in animal cells for applications in medicine and structural biology
BIO-701 / 2 crédits
Enseignant(s): Abriata Luciano Andres, Hacker David, Lau Kelvin Ka Ching, Pojer Florence
Langue: Anglais
Remark: Every year
Frequency
Every 2 years
Summary
Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins.
Content
Subjects discussed in class are expected to include:
- Transient gene expression
- Stable gene expression
- Cell-free protein synthesis
- Production of therapeutic antibodies
- Production of transmembrane proteins
- X-ray structure determination
- Cryogenic electron microscopy
- Bio-NMR
- Biophysical techniques for protein characterization
Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.
Note
This course will take place in autumn 2025, exact dates and place to be confirmed.
Max. 20 participants. To register, please send an email to edms@epfl.ch
Keywords
recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure
Learning Prerequisites
Required courses
Basic cell biology
Learning Outcomes
By the end of the course, the student must be able to:
- Explain (i.e. comprehend) technologies discussed in course
- Interpret in a critical way data generated with these technologies
Assessment methods
Oral presentation
Dans les plans d'études
- Nombre de places: 15
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
- Cours: 28 Heure(s)