BIO-701 / 2 crédits

Enseignant(s): Abriata Luciano Andres, Duhoo Yoan Désiré, Hacker David, Lau Kelvin Ka Ching, Pojer Florence

Langue: Anglais

Remark: Every year


Summary

Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins.

Content

 

  • Transient gene expression
  • Stable gene expression
  • Prodcution in insect and bacterial cells
  • Cell-free protein synthesis
  • Production of therapeutic antibodies
  • Production of transmembrane proteins
  • X-ray structure determination
  • Cryogenic electron microscopy
  • Bio-NMR
  • Biophysical techniques for protein characterization


Other topics may be included as needed.

Keywords

recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure

Learning Prerequisites

Required courses

Basic knowledge in cell biology

Learning Outcomes

By the end of the course, the student must be able to:

  • Explain biotechnologies discussed in the course
  • Interpret data generated

Teaching methods

Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region.

Assessment methods

Oral presentation on a specific subject within the field

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Dans les plans d'études

  • Nombre de places: 15
  • Forme de l'examen: Exposé (session libre)
  • Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
  • Cours: 28 Heure(s)
  • Type: optionnel
  • Nombre de places: 15
  • Forme de l'examen: Exposé (session libre)
  • Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
  • Cours: 28 Heure(s)
  • Type: optionnel

Semaine de référence

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