Recombinant protein expression in animal cells for applications in medicine and structural biology
BIO-701 / 2 crédits
Enseignant(s): Abriata Luciano Andres, Duhoo Yoan Désiré, Hacker David, Lau Kelvin Ka Ching, Pojer Florence
Langue: Anglais
Remark: Every year
Summary
Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins.
Content
- Transient gene expression
- Stable gene expression
- Prodcution in insect and bacterial cells
- Cell-free protein synthesis
- Production of therapeutic antibodies
- Production of transmembrane proteins
- X-ray structure determination
- Cryogenic electron microscopy
- Bio-NMR
- Biophysical techniques for protein characterization
Other topics may be included as needed.
Note
This course takes place every 1.5 year! As it took place in Spring 2025, it won't take place before Autumn 2026!
Keywords
recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure
Learning Prerequisites
Required courses
Basic knowledge in cell biology
Learning Outcomes
By the end of the course, the student must be able to:
- Explain biotechnologies discussed in the course
- Interpret data generated
Teaching methods
Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region.
Assessment methods
Oral presentation on a specific subject within the field
.
Resources
Websites
Moodle Link
Dans les plans d'études
- Nombre de places: 15
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
- Cours: 28 Heure(s)
- Type: optionnel
- Nombre de places: 15
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
- Cours: 28 Heure(s)
- Type: optionnel