BIO-701 / 2 crédits
Remark: Every year
Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins from animal cells.
Subjects discussed in class are expected to include:
- Transient gene expression
- Stable gene expression
- Cell-free protein synthesis
- X-ray diffraction
- Cryogenic electron microscopy
- Production of therapeutic antibodies
- Production of transmembrane proteins
- Biophysical techniques for protein characterization
Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.
Next session for this course : autumn 2021, from 21st September to 21st December, room AI 1153.
Taking place every Tuesdays from 3 to 5pm, depending on the COVID sanitary situation by then.
Max. 15 participants. To register, please send an email to firstname.lastname@example.org
recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure
Basic cell biology
By the end of the course, the student must be able to:
- Explain (i.e. comprehend) technologies discussed in course
- Interpret in a critical way data generated with these technologies
Dans les plans d'études
- Nombre de places: 15
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
- Cours: 28 Heure(s)