Practical - Stahlberg Lab

BIO-603(SH) / 1 crédit

Enseignant: Stahlberg Henning Paul-Julius

Langue: Anglais

Remark: 3-day Block course, every year in January. To register, contact EDMS

Frequency

Every 2 years

Summary

Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.

Content

Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.

Note

Please note that you cannot register in your own group Practical!

Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.

Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!

Keywords

Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction

Resources

Websites

Dans les plans d'études

  • Nombre de places: 3
  • Forme de l'examen: Exposé (session libre)
  • Matière examinée: Practical - Stahlberg Lab
  • Cours: 6 Heure(s)
  • Exercices: 6 Heure(s)
  • TP: 16 Heure(s)
  • Type: obligatoire

Semaine de référence

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