BIO-603(SH) / 1 crédit
Enseignant: Stahlberg Henning Paul-Julius
Remark: 3-day Block course, every year in January. To register, contact EDMS
Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.
Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.
Please note that you are not allowed to inscribe in your own group!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction
Dans les plans d'études
- Nombre de places: 3
- Forme de l'examen: Exposé (session libre)
- Matière examinée: Practical - Stahlberg Lab
- Cours: 6 Heure(s)
- Exercices: 6 Heure(s)
- TP: 16 Heure(s)