Mass spectrometry, principles and applications
CH-728 / 3 crédits
Enseignant(s): Gasilova Natalia, Menin Laure, Ortiz Trujillo Daniel, Patiny Luc
Langue: Anglais
Remark: Next time: Fall 2023. Pre-registration form https://docs.google.com/forms/d/e/1FAIpQLSd5lyrEGUar8FBwxPqnIW1JBoPBr4YQjiA1yRGt3E7kOL-nXQ/viewform
Frequency
Every year
Summary
The goal is to provide students with a complete overview of the principles and key applications of modern mass spectrometry and meet the current practical demand of EPFL researchers to improve structural analsis of molecules. Numerous instrumental aspects of mass spectrometry are described.
Content
The course program includes:
Week 1
1. Lectures
1.1 General introduction to MS: Definitions/Instrumentation
1.2 MS/MS:fragmentation methods and mechanisms; Ion Mobility MS
1.3 LC-MS and other hyphenated techniques
1.4 ICP-MS
1.5 Summary of all concepts and fundamental MS aspects seen during the week.
2. Exercises:
Ms.cheminfo org tools for advanced MS
3. Pratical work:
3.1 Fragmentation of small molecules (sugars, small peptides) using QTOF and FT-Orbitrap- MS, fragmentation by EI; interpretation of mass spectra using different tools and softwares.
3.2 Individual work for each doctorant topics.
Week 2
1. Lectures
1.1 High Resolution Mass spectrometry
1.2 Photo-dissociation spectroscopy mass spectrometry
1.3 Peptidomics/Proteomics: top-down and bottom-up approaches
1.4 MALDI-TOF: principles and applications
1.5 Ion Mobility MS
2. Exercises
Exercices on all topics covered by the MS course
3. Final exam
15'presentation of each PhD student of its individual Practical Work
The course includes practical work in mass spectrometry that will be given in the Mass Spectrometry Service Facility of ISIC (SSMI, SB, EPFL).
Note
Pre-enrolment form
Keywords
mass spectrometry, tandem mass spectrometry, High-resolution mass spectrometry (HRMS), liquid chromatography, Gas chromatography, quantification, proteomics, lipidomics, metabolomics, proteomics
Assessment methods
Oral
Resources
Websites
Moodle Link
Dans les plans d'études
- Nombre de places: 10
- Forme de l'examen: Oral (session libre)
- Matière examinée: Mass spectrometry, principles and applications
- Cours: 20 Heure(s)
- Exercices: 8 Heure(s)
- TP: 24 Heure(s)