CH-319 / 4 crédits

Enseignant: Hovius Rudolf

Langue: Anglais

Withdrawal: It is not allowed to withdraw from this subject after the registration deadline.


Summary

A semester long experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from bacteria, and characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper.

Content

Keywords

Molecular biology - cloning

Protein expression & purification

Bio-chemical and bio-physical chracterisation

Sensor proteins

Reporting

Learning Prerequisites

Required courses

Biochimie I (CH-210);

Chemistry practicals

 

Recommended courses

Molecular and cellular biophysics I (CH-311)

Important concepts to start the course

genetic engineering & DNA manipulation; protein synthesis; DNA & protein analysis; absorbance and fluorescence spectrometry; enzymology, molecular interactions

Learning Outcomes

  • Design cloning strategy
  • Produce a scientific report and high-quality lab journal
  • Integrate Good laboratory behavior and wet lab practice
  • Assess / Evaluate your data critically
  • Produce a purified expressed protein
  • Analyze proteins and DNA
  • Characterize sensor function
  • Use common sense and logical deduction
  • Interpret protein structure data

Transversal skills

  • Plan and carry out activities in a way which makes optimal use of available time and other resources.
  • Collect data.
  • Continue to work through difficulties or initial failure to find optimal solutions.
  • Write a scientific or technical report.

Teaching methods

Students prepare and discuss experimental approach

Laboratory experimentation

Discussion of experimental progress and results

Reporting on scientific level

Topiocal presentations

Expected student activities

Good theoretical preparation & planning of lab work before doing experiments; skillful execution of experiments, being organized & keeping a lab book; thorough analysis of results, writting a scientific-grade report; respecting security rules and fellow students.

 

Assessment methods

Evaluation of preparation and planning

Evaluation of experimental execution, including good laboratory behaviour

Evaluation of comprehension through discussion & written questions

Evaluation of report: including structure, data treatment and presentation, critical attitude , comparison to scientific literature

 

Supervision

Assistants Yes
Others

Resources

Bibliography

TP manual

Biochemistry & Biophysics text books

ApE; a plasmid editor free software

Methods, Structures, and other useful info via Moodle

Websites

Prerequisite for

A big plus for Msc courses and for semester or diploma projects in chemical biology or biophysics

 

Dans les plans d'études

  • Semestre: Printemps
  • Forme de l'examen: Pendant le semestre (session d'été)
  • Matière examinée: Experimental biochemistry and biophysics
  • TP: 6 Heure(s) hebdo x 14 semaines
  • Semestre: Printemps
  • Forme de l'examen: Pendant le semestre (session d'été)
  • Matière examinée: Experimental biochemistry and biophysics
  • TP: 6 Heure(s) hebdo x 14 semaines

Semaine de référence

 LuMaMeJeVe
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22