Fiches de cours

Chemical biology of cell imaging

CH-416

Enseignant(s) :

Rivera Fuentes Pablo

Langue:

English

Summary

This course will introduce basic concepts of fluorescence spectroscopy and microscopy applied to the observation of biological systems. The course will focus on the design, preparation and implementation of small-molecule and protein-based probes.

Content

Principles of fluorescence spectroscopy and microscopy, fluorescent dyes and proteins, chemiluminescence, super-resolution microscopy, and fluorescent sensors.

Keywords

fluorescence, labeling, imaging, cell biology, photophysical properties, proteins, sensors

Learning Prerequisites

Recommended courses

Basic chemistry and biology courses

Important concepts to start the course

Electronic absorption and fluorescence; molecular orbitals; basic concepts of electron donating and accepting funtional groups; structure of proteins, basic understanding of the central dogma of molecular biology, basic cell biology.

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

In this course, students learn to design efficient fluorescence bioimaging experiments. To achieve this objective, a combination of teaching methods is implemented, including regular lectures, case studies, analysis of recent papers, and design of experiments for ¿real-world¿ problems. Constant work in small teams and ungraded weekly quizzes are implemented to help students learn by themselves and manage large amounts of multidisciplinary information throughout the course.

Expected student activities

Lecture attendance, participation in interactive sequences, design and presentation of a poster, participation in weekly online quizzes, participation in a final oral presentatation at the end of the semester, if applicable, participation in instrument demonstrations.

Assessment methods

Oral exam. Oral presentation and weekly quizzes provide additional points.

Supervision

Office hours No
Assistants Yes
Forum No
Others Although no official office hours are provided, students are always welcome to send their question by e-mail, Moodle, or ask in person before, during and after class.

Resources

Bibliography

J. R. Lakowicz. Principles of Fluorescence Spectroscopy. Kluwer Academic / Plenum Publishers. 2006.

P. J. Walla. Modern Biophysical Chemistry: Detection and Analysis of Biomolecules. Wiley-VCH. 2014.

M. Chalfie; S. R. Kain (Eds.) Green Fluorescent Protein: Properties, Applications, and Protocols.
Wiley-Interscience. 2006.

R. W. Sabnis. Handbook of Fluorescent Dyes and Probes. John Wiley & Sons, Inc. 2015.

A. P. Demchenko. Introduction to Fluorescence Sensing. Springer Science. 2009.

Ressources en bibliothèque
Notes/Handbook

Slides and handouts will be available on Moodle before class.

Moodle Link

Dans les plans d'études

  • Chimie moléculaire et biologique, 2019-2020, Master semestre 1
    • Semestre
      Automne
    • Forme de l'examen
      Oral
    • Crédits
      3
    • Matière examinée
      Chemical biology of cell imaging
    • Cours
      3 Heure(s) hebdo x 14 semaines
  • Chimie moléculaire et biologique, 2019-2020, Master semestre 3
    • Semestre
      Automne
    • Forme de l'examen
      Oral
    • Crédits
      3
    • Matière examinée
      Chemical biology of cell imaging
    • Cours
      3 Heure(s) hebdo x 14 semaines

Semaine de référence

LuMaMeJeVe
8-9
9-10
10-11CE1105
11-12
12-13
13-14
14-15
15-16
16-17
17-18
18-19
19-20
20-21
21-22
Cours
Exercice, TP
Projet, autre

légende

  • Semestre d'automne
  • Session d'hiver
  • Semestre de printemps
  • Session d'été
  • Cours en français
  • Cours en anglais
  • Cours en allemand