BIO-212 / 3 crédits
Biochemistry is a key discipline in the Life Sciences. Biological Chemistry I and II are two tightly interconnected courses that aims to understand in molecular terms the processes that make life possible.
We need to understand biological macromolecules at the atomic level, as well as their energetic properties and interplay between them. With these insights, we will dissect basic macromolecular structure and how it translates into biological function. Among the major types of biological macromolecules, including nucleic acids, proteins, lipids and glycans, we will put a main emphasis on proteins - the workhorses of cells.
- Building blocks: Sugars, lipids, amino acids
- Protein structural organization, folding
- Analysis of proteins
- Protein expression and purification
- Advanced protein purification, X-ray crystallography
- Protein structure determination by NMR and cryo-EM
- Visualization and analysis of protein structures using Pymol
- Energy and intramolecular forces in proteins
- Free energy, thermodynamics, kinetics
- Methods to measure protein-protein and protein ligand interactions.
- Enzymatic catalysis and reaction mechanisms
- Enzyme kinetics
roteins, lipids, carbohydrates, nucleic acids, structural biology, biochemistry, enzymes, experimental methods, protein folding, biophysics
General Biology, Organic chemistry
Important concepts to start the course
- Basic understanding of chemical matter
- Basic understanding of reaction kinetics
- Basic understanding of thermodynamics
- Basic reaction mechanisms in organic chemistry
By the end of the course, the student must be able to:
- Design experimental workflows for biochemical analysis
- Formalize reaction mechanisms of biological macromolecules
- Formulate questions that adress important biological problems
- Integrate diverse sources of data
- Generalize principles applicable to wide variety of biological problems
- Assess one's own level of skill acquisition, and plan their on-going learning goals.
- Continue to work through difficulties or initial failure to find optimal solutions.
- Demonstrate the capacity for critical thinking
- Manage priorities.
- Exercise session accompanying each lecture
- Execises session on bioinformatics and proteins structure visualization tools
Expected student activities
- Attending to classes
- Attendance to exercise
- Class participation
- written exam
Virtual desktop infrastructure (VDI)
- Kuriyan/Konforti/Wemmer - The Molecules of Life
Ressources en bibliothèque
Dans les plans d'études
- Semestre: Automne
- Forme de l'examen: Ecrit (session d'hiver)
- Matière examinée: Biological chemistry I
- Cours: 2 Heure(s) hebdo x 14 semaines
- Exercices: 1 Heure(s) hebdo x 14 semaines