Recombinant protein expression in animal cells for appli-cations in medicine and structural biology
BIO-701 / 2 credits
Teacher(s): Hacker David, Pojer Florence, Abriata Luciano Andres, Lau Kelvin Ka Ching
Language: English
Remark: Every year
Frequency
Every year
Summary
Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins from animal cells.
Content
Subjects discussed in class are expected to include:
• Transient gene expression
• Stable gene expression
• Cell-free protein synthesis
• X-ray diffraction
• Cryogenic electron microscopy
• Production of therapeutic antibodies
• Production of transmembrane proteins
• NMR
• Biophysical techniques for protein characterization
Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.
Note
Next session for this course : autumn 2020
Starting on Tuesday 15th September and ending on 15th December, taking place every Tuesdays from 3 to 5pm either in room AI 2142 or by Zoom, depending on the COVID sanitary situation by then.
Max. 15 participants. To register, please send an email to edms@epfl.ch
Keywords
recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure
Learning Prerequisites
Required courses
Basic cell biology
Learning Outcomes
By the end of the course, the student must be able to:
- Explain (i.e. comprehend) technologies discussed in course
- Interpret in a critical way data generated with these technologies
Assessment methods
Oral presentation
In the programs
- Exam form: Oral presentation (session free)
- Subject examined: Recombinant protein expression in animal cells for appli-cations in medicine and structural biology
- Lecture: 28 Hour(s)
Reference week
| Mo | Tu | We | Th | Fr | |
| 8-9 | |||||
| 9-10 | |||||
| 10-11 | |||||
| 11-12 | |||||
| 12-13 | |||||
| 13-14 | |||||
| 14-15 | |||||
| 15-16 | |||||
| 16-17 | |||||
| 17-18 | |||||
| 18-19 | |||||
| 19-20 | |||||
| 20-21 | |||||
| 21-22 |
Légendes:
Lecture
Exercise, TP
Project, other