BIO-701 / 2 credits

Teacher(s): Hacker David, Pojer Florence, Abriata Luciano Andres, Lau Kelvin Ka Ching

Language: English

Remark: Every year


Frequency

Every year

Summary

Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins from animal cells.

Content

Note

Next session for this course : autumn 2020

Starting on Tuesday 15th September and ending on 15th December, taking place every Tuesdays from 3 to 5pm either in room AI 2142 or by Zoom, depending on the COVID sanitary situation by then.

Max. 15 participants. To register, please send an email to edms@epfl.ch

Keywords

recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure

Learning Prerequisites

Required courses

Basic cell biology

Learning Outcomes

By the end of the course, the student must be able to:

  • Explain (i.e. comprehend) technologies discussed in course
  • Interpret in a critical way data generated with these technologies

Assessment methods

Oral presentation

In the programs

  • Exam form: Oral presentation (session free)
  • Subject examined: Recombinant protein expression in animal cells for appli-cations in medicine and structural biology
  • Lecture: 28 Hour(s)

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22