Coursebooks

Recombinant protein expression in animal cells for appli-cations in medicine and structural biology

BIO-701

Lecturer(s) :

Abriata Luciano Andres
Hacker David
Lau Kelvin Ka Ching
Pojer Florence

Language:

English

Frequency

Every year

Remarque

Every 2 years

Summary

Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins from animal cells.

Content

Subjects discussed in class are expected to include:

¿ Transient gene expression

¿ Stable gene expression

¿ Cell-free protein synthesis

¿ X-ray diffraction

¿ Cryogenic electron microscopy

¿ Production of therapeutic antibodies

¿ Production of transmembrane proteins

¿ NMR

¿ Biophysical techniques for protein characterization

Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.

Keywords

recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure

Learning Prerequisites

Required courses

Basic cell biology

Learning Outcomes

By the end of the course, the student must be able to:

• Explain (i.e. comprehend) technologies discussed in course
• Interpret in a critical way data generated with these technologies

Assessment methods

Oral presentation