CH-419 / 2 credits

Teacher: Dayon Loïc

Language: English


Summary

In systems biology, proteomics represents an essential pillar. The understanding of protein function and regulation provides key information to decipher the complexity of living systems. Proteomic technology now enables deep quantitative proteome mapping via mass spectrometry.

Content

Keywords

Mass Spectrometry; Liquid Chromatography; Electrophoresis; Proteins; Peptides; Systems Biology; Bioinformatics; Human Samples; Clinics; Isotopic Labelling; Quantification

Learning Prerequisites

Recommended courses

CH-210 Biochimie I

CH-301 Méthodes de séparation analytiques

CH-405 Methodology in instrumental chromatography

CH-403 Mass spectrometry

Important concepts to start the course

Mass spectrometry; Biochemistry

Learning Outcomes

By the end of the course, the student must be able to:

  • Differentiate biological mass spectrometry techniques to identify and quantify proteomes
  • Distinguish the main separation techniques for proteins and peptides
  • Choose appropriate methodology for protein analysis
  • Discuss literature related to proteomics
  • Describe the field of applications of proteomics and expand those concepts beyond, e.g., to metabolomics
  • Interpret some mass spectrometry data

Teaching methods

Ex-cathedra

Assessment methods

Written exam with Multiple Choice Questions (MCQ) (75%) & exercices (25%)

[Oral presentation of a paper/study by group of 2-3 students may be an option depending on the Covid-19 situation]

Supervision

Office hours No
Assistants No
Forum No

In the programs

  • Semester: Spring
  • Exam form: Written (summer session)
  • Subject examined: Protein mass spectrometry and proteomics
  • Lecture: 2 Hour(s) per week x 14 weeks
  • Semester: Spring
  • Exam form: Written (summer session)
  • Subject examined: Protein mass spectrometry and proteomics
  • Lecture: 2 Hour(s) per week x 14 weeks
  • Semester: Spring
  • Exam form: Written (summer session)
  • Subject examined: Protein mass spectrometry and proteomics
  • Lecture: 2 Hour(s) per week x 14 weeks

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22