Coursebooks

Practical - Stahlberg Lab

BIO-603(SH)

Lecturer(s) :

Stahlberg Henning Paul-Julius

Language:

English

Frequency

Every year

Remark

3-day Block course, every year in January. To register, contact EDMS

Summary

Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.

Content

Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.

Note

Open to max. 3 students. Please note that you are not allowed to inscribe in your own group!

Keywords

Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction

Resources

Websites

In the programs

    • Semester
    • Exam form
       Oral presentation
    • Credits
      1
    • Subject examined
      Practical - Stahlberg Lab
    • Number of places
      3
    • Lecture
      6 Hour(s)
    • Exercises
      6 Hour(s)
    • Practical work
      16 Hour(s)

Reference week

 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German