BIO-603(SH) / 1 credit
Teacher: Stahlberg Henning Paul-Julius
Remark: 3-day Block course, every year in January. To register, contact EDMS
Cryo-electron microscopy structural analysis of proteins. The course aims at demonstrating the workflow from sample purification to determining the atomic structure of a soluble or membrane protein.
Students will be introduced to the principles of protein purification, biophysical characterization of the state of the proteins, and structure determination by cryo-EM. The latter will involve sample preparation for negative stain EM and cryo-EM, image recording in an advanced and automated electron microscope, image processing, and 3D structure reconstruction.
Open to max. 3 students. Please note that you are not allowed to inscribe in your own group!
Structural biology, membrane protein structure, cryo-electron microscopy, image processing, 3D reconstruction
In the programs
- Exam form: Oral presentation (session free)
- Subject examined: Practical - Stahlberg Lab
- Lecture: 6 Hour(s)
- Exercises: 6 Hour(s)
- Practical work: 16 Hour(s)