BIO-622 / 1 credit
Teacher: Lingner Joachim
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Telomere biology. The students will obtain theoretical and practical insight into telomere biology and the roles of telomeres during cellular senescence and for genome stability.
A general theoretical introduction will be given in the beginning of the course. In the laboratory, human cells will be used as model systems. Biochemical, molecular biological and cell biological assays will be performed. Specifically, telomerase activity will be measured in cellular extracts, the affinity of an RNA-protein interaction will be determined in band-shift assays and telomere integrity will be assessed by immunofluorescence.
Telomeres, telomerase, reverse transcriptase, cellular senescence, genome stability
Background reading: Nandakumar, J and Cech, TR: Finding the end: recruitment of telomerase to telomeres. Nat Rev Mol Cell Biol 2013 Feb; 14(2):69-82
In the programs
- Exam form: Oral presentation (session free)
- Subject examined: Practical - Lingner Lab
- Lecture: 5 Hour(s)
- Practical work: 21 Hour(s)