Coursebooks

Practical - Dyson Lab

BIO-603(DP)

Lecturer(s) :

Dyson Paul Joseph

Language:

English

Frequency

Every year

Remark

3-day Block course, every year in January. To register, contact EDMS Administration

Summary

In vitro cytotoxicity testing is often the first step to establish the utility of a compound as a potential drug. The course will teach students how to evaluate the cytotoxicity of compounds on cancer cells of human-origin and appropriate non-tumorigenic cell lines.

Content

In the course, the MTT cell viability assay will be used to determine compound sensitivity profiles and to estimate the efficacy of potential chemotherapeutic compounds as drugs.

 

Preparation, treatment and analysis:

 

- Plating cancer and non-tumorigenic cells
- Preparation of compound dilutions
- Cell treatment protocols
- Determination of the cell viability
- Spectrophotometric analysis
- Preparing compound sensitivity profiles
- Estimation of errors and statistical analysis

Note

Open to max. 4 students. Please note that you are not allowed to inscribe in your own group!

Keywords

Cancer; chemotherapy; drug screening

Learning Outcomes

By the end of the course, the student must be able to:

• Assess / Evaluate the cytotoxicity of a compound

Assessment methods

Oral presentation

Resources

Websites

• https://www.epfl.ch/labs/lcom/