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Practical - Dyson Lab
BIO-603(DP)
Lecturer(s) :
Dyson Paul JosephLanguage:
English
Frequency
Every yearRemark
3-day Block course, every year in January. To register, contact EDMS AdministrationSummary
In vitro cytotoxicity testing is often the first step to establish the utility of a compound as a potential drug. The course will teach students how to evaluate the cytotoxicity of compounds on cancer cells of human-origin and appropriate non-tumorigenic cell lines.Content
In the course, the MTT cell viability assay will be used to determine compound sensitivity profiles and to estimate the efficacy of potential chemotherapeutic compounds as drugs.
Preparation, treatment and analysis:
- Plating cancer and non-tumorigenic cells
- Preparation of compound dilutions
- Cell treatment protocols
- Determination of the cell viability
- Spectrophotometric analysis
- Preparing compound sensitivity profiles
- Estimation of errors and statistical analysis
Note
Open to max. 4 students. Please note that you are not allowed to inscribe in your own group!
Keywords
Cancer; chemotherapy; drug screening
Learning Outcomes
By the end of the course, the student must be able to:- Assess / Evaluate the cytotoxicity of a compound
Assessment methods
Oral presentation
Resources
Websites
In the programs
- Semester
- Exam form
Oral presentation - Credits
1 - Subject examined
Practical - Dyson Lab - Number of places
4 - Lecture
6 Hour(s) - Practical work
18 Hour(s)
- Semester
Reference week
Lecture
Exercise, TP
Project, other
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- Autumn semester
- Winter sessions
- Spring semester
- Summer sessions
- Lecture in French
- Lecture in English
- Lecture in German