Coursebooks

Practical - Blokesch Lab

BIO-666

Lecturer(s) :

Blokesch Melanie

Language:

English

Frequency

Every year

Remarque

3-day Block course, every year in January. To register, contact EDMS Administration

Summary

Next time in January 2020 or 2021 (depending on number of new PhD students in the EDMS program). How to look at tiny things: visualizing protein localization in bacteria using epifluorescence microscopy.

Content

Theory:
Basics on protein localization in bacteria.


Practical part:
Preparing bacteria for microscopy.
Staining methods to visualize bacteria.
Epifluorescence microscopy.
Comparison of protein localization depending on protein levels (varying artifical induction).
Basic image analysis (MicrobeTracker).
Independent analysis of an unknown bacterium for cell shape and potential localized fluorescently labeled proteins.

Assessment methods

Oral presentation

Resources

Websites

In the programs

Reference week

 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German