Practical - Blokesch Lab
BIO-666 / 1 credit
Teacher: Blokesch Melanie
Language: English
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
How to look at tiny things: visualizing protein localization in bacteria using epifluorescence microscopy.
Content
Theory:
- Basics on protein localization in bacteria.
Practical part:
- Preparing bacteria for microscopy.
- Staining methods to visualize bacteria.
- Epifluorescence microscopy.
- Comparison of protein localization depending on protein levels (varying artifical induction).
- Basic image analysis (MicrobeTracker).
- Independent analysis of an unknown bacterium for cell shape and potential localized fluorescently labeled proteins.
Note
Please note that you are not allowed to inscribe in your own group!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Assessment methods
Oral presentation
In the programs
- Number of places: 4
- Exam form: Oral presentation (session free)
- Subject examined: Practical - Blokesch Lab
- Lecture: 6 Hour(s)
- Practical work: 18 Hour(s)