Coursebooks

Practical - Barth Lab

BIO-603(BP)

Lecturer(s) :

Barth Patrick Daniel
Oggier Aurélien Laurent Jean-Charles

Language:

English

Frequency

Every year

Remark

3-day Block course, every year in January. To register, contact EDMS Administration

Summary

This course will convey the concepts and experimental techniques for studying the signal transduction mediated by receptors across biological membranes.

Content

The course will introduce the concepts and technical approaches for studying signal transduction pathways mediated by recpetors across biological membranes.

 

On the conceptual side, we will cover the molecular and mechanistic underpinnings of:

 

receptor ligand sensing and binding selectivity
receptor allostery and signal transmission
receptor coupling to intracellular signaling proteins
intracellular signaling cascades and associated protein networks.

 

On the practical side, the following techniques will be introduced:

 

mamallian cell culture, transfections
quantitative measurements of receptor and downstream signaling pathway activations using specific reporters of secondary messenger production and gene expression
Fluorescence, Bioluminescence meaurements using plate readers and microscopy.

Note

Open to max. 4 students. Please note that you are not allowed to inscribe in your own group!

Keywords

cell signaling; bioluminescence, fluorescence, biosensors

Learning Outcomes

By the end of the course, the student must be able to:

Assessment methods

Oral

In the programs

Reference week

 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German