BIO-603(BP) / 1 credit
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
This course will convey the concepts and experimental techniques for studying the signal transduction mediated by receptors across biological membranes.
The course will introduce the concepts and technical approaches for studying signal transduction pathways mediated by recpetors across biological membranes.
On the conceptual side, we will cover the molecular and mechanistic underpinnings of:
receptor ligand sensing and binding selectivity
receptor allostery and signal transmission
receptor coupling to intracellular signaling proteins
intracellular signaling cascades and associated protein networks.
On the practical side, the following techniques will be introduced:
mamallian cell culture, transfections
quantitative measurements of receptor and downstream signaling pathway activations using specific reporters of secondary messenger production and gene expression
Fluorescence, Bioluminescence meaurements using plate readers and microscopy.
Open to max. 4 students. Please note that you are not allowed to inscribe in your own group!
cell signaling; bioluminescence, fluorescence, biosensors
By the end of the course, the student must be able to:
- measure and understand the signaling properties of a membrane receptor
In the programs
- Exam form: Oral (session free)
- Subject examined: Practical - Barth Lab
- Lecture: 6 Hour(s)
- Practical work: 18 Hour(s)