Practical - Barth Lab
BIO-603(BP) / 1 credit
Teacher(s): Barth Patrick Daniel, Oggier Aurélien Laurent Jean-Charles
Language: English
Remark: 3-day Block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
This course will convey the concepts and experimental techniques for studying the signal transduction mediated by receptors across biological membranes.
Content
The course will introduce the concepts and technical approaches for studying signal transduction pathways mediated by recpetors across biological membranes.
On the conceptual side, we will cover the molecular and mechanistic underpinnings of:
receptor ligand sensing and binding selectivity
receptor allostery and signal transmission
receptor coupling to intracellular signaling proteins
intracellular signaling cascades and associated protein networks.
On the practical side, the following techniques will be introduced:
mamallian cell culture, transfections
quantitative measurements of receptor and downstream signaling pathway activations using specific reporters of secondary messenger production and gene expression
Fluorescence, Bioluminescence meaurements using plate readers and microscopy.
Note
Please note that you are not allowed to inscribe in your own group!
Note that 3 practical courses are mandatory for all EDMS students and that they have the priority; each course has between 2 to 4 possible slots.
Therefore, please do not register by yourself to this course, this will be done by the EDMS program administrator!
Keywords
cell signaling; bioluminescence, fluorescence, biosensors
Learning Outcomes
By the end of the course, the student must be able to:
- measure and understand the signaling properties of a membrane receptor
Assessment methods
Oral
In the programs
- Number of places: 4
- Exam form: Oral (session free)
- Subject examined: Practical - Barth Lab
- Lecture: 6 Hour(s)
- Practical work: 18 Hour(s)