Coursebooks

Experimental biochemistry and biophysics

CH-319

Lecturer(s) :

Hovius Rudolf

Language:

English

Withdrawal

It is not allowed to withdraw from this subject after the registration deadline.

Summary

A semester long experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from bacteria, and characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper.

Content

i) Molecular biology: DNA fragment isolation by PCR, DNA restriction and ligation, plasmid purification and sequence analysis, agarose gel electrophoresis.

ii) Biochemistry: Bacterial protein expression, protein purification by affinity chromatography, analysis by SDS-PAGE and spectroscopy, fluorescent labelling

iii) Biophysics: Fluorescence spectroscopy, enzyme kinetics or molecular interactions.

 

Keywords

Molecular biology - cloning

Protein expression & purification

Bio-chemical and bio-physical chracterisation

Sensor proteins

Reporting

Learning Prerequisites

Required courses

Biochimie I (CH-210);

Chemistry practicals

 

Recommended courses

Molecular and cellular biophysics I (CH-311)

Important concepts to start the course

genetic engineering & DNA manipulation; protein synthesis; DNA & protein analysis; absorbance and fluorescence spectrometry; enzymology, molecular interactions

Learning Outcomes

Transversal skills

Teaching methods

Students prepare and discuss experimental approach

Laboratory experimentation

Discussion of experimental progress and results

Reporting on scientific level

Topiocal presentations

Expected student activities

Good theoretical preparation & planning of lab work before doing experiments; skillful execution of experiments, being organized & keeping a lab book; thorough analysis of results, writting a scientific-grade report; respecting security rules and fellow students.

 

Assessment methods

Evaluation of preparation and planning

Evaluation of experimental execution, including good laboratory behaviour

Evaluation of comprehension through discussion & written questions

Evaluation of report: including structure, data treatment and presentation, critical attitude , comparison to scientific literature

 

Supervision

Assistants Yes
Others

Discussions are possible at office hours, depending on the availability of assistants and lecturer,

Experimentation might be possible at office hours, depending on the availability of assistants and lecturer, and only upon the allowance of lecturer.

 

Resources

Bibliography

TP manual

Biochemistry & Biophysics text books

ApE; a plasmid editor free software

Methods, Structures, and other useful info via Moodle

Websites

Prerequisite for

A big plus for Msc courses and for semester or diploma projects in chemical biology or biophysics

 

In the programs

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
Under construction
 
      Lecture
      Exercise, TP
      Project, other

legend

  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German