Biotechnology lab (for CGC)
ChE-433 / 4 credits
Teacher: Pick Horst
Language: English
Withdrawal: It is not allowed to withdraw from this subject after the registration deadline.
Summary
This laboratory-training course is designed to give students a comprehensive insight into laboratory research techniques in the field of biotechnology and pharmaceutical biotechnology with practices employed in the industry. Accompanying lectures provide the theoretical background.
Content
- Engineering of a synthetic gene encoding human pro-insulin using recombinant DNA techniques
- Basic animal/human cell culture training (Aseptic techniques, quantification of cell growth, cell passaging, adherent and suspension cells cultures, determination of cell viability).
- Engineering of a stable mammalian cell line using a transposon-based approach (Production of a recombinant protein for the treatment of rheumatoid arthritis).
- Bioreactor training for pharmaceutical protein production (Fed-batch culture, daily sampling for analysis: cell density, viability, glucose, lactate, glutamine).
- Pharmaceutical protein purification, SDS-page, ELISA, Mass spectrometry analysis.
- Production and purification of an anti-Rhesus D monoclonal antibody.
- Production and purification of a recombinant anti-tuberculosis vaccine.
Keywords
Cell culture technologies, therapeutic proteins, monoclonal antibodies, recombinant vaccines, bioreactor, affinity purification, ELISA, mass spectrometry, flow cytometry, recombinant DNA techniques.
Learning Prerequisites
Required courses
Recommended courses:
Pharmaceutical Biotechnology (ChE-436)
Learning Outcomes
By the end of the course, the student must be able to:
- Demonstrate comprehensive understanding of the principles and practices of biotechnology
- Demonstrate technical skills in methods of biotechnology
- Manage working in a team to organise laboratory activities
- Choose an appropriate method for obtaining experimental data
- Demonstrate the safe use and disposal of chemicals and biological samples
- Demonstrate the development of analytical skills for the management and communication of experimental data
- Demonstrate understanding of typical conventions of lab or experimental reports
- Elaborate good scientific writing, appropriate use of scientific literature and scientific data presentation (Figures/tables)
Transversal skills
- Plan and carry out activities in a way which makes optimal use of available time and other resources.
- Set objectives and design an action plan to reach those objectives.
Teaching methods
Lectures, laboratory training
Expected student activities
Hands-on lab experiments, lab reports
Assessment methods
During the semester
- Three written tests (20 %)
- Lab participation (10 %)
- Three written lab reports (70 %)
Supervision
| Office hours | Yes |
| Assistants | Yes |
| Forum | Yes |
In the programs
- Semester: Spring
- Exam form: During the semester (summer session)
- Subject examined: Biotechnology lab (for CGC)
- TP: 6 Hour(s) per week x 14 weeks
- Type: optional
- Semester: Spring
- Exam form: During the semester (summer session)
- Subject examined: Biotechnology lab (for CGC)
- TP: 6 Hour(s) per week x 14 weeks
- Type: optional
- Semester: Spring
- Exam form: During the semester (summer session)
- Subject examined: Biotechnology lab (for CGC)
- TP: 6 Hour(s) per week x 14 weeks
- Type: optional