BIOENG-421 / 4 credits

Teacher: Merten Christoph

Language: English

Withdrawal: It is not allowed to withdraw from this subject after the registration deadline.


Summary

This combined practical and theoretical course will provide the basics in bioinstrumentation, including the construction of a droplet-microfluidic workstation for high-throughput, single-cell analysis. Many of the learned concepts are equally applicable to other widely used lab instruments.

Content

Keywords

  • High throughput fluorescence analysis
  • Single-cell assays
  • Microfluidics
  • System engineering
  • Hands-on practice
  • LabVIEW control software

Learning Prerequisites

Required courses

none

Learning Outcomes

By the end of the course, the student must be able to:

  • Design an instrument converting a cellular feature (e.g. an enzymatic activity or the presence of a surface receptor) into an electric signal that can be quantified and processed to provide active feedback loops (e.g. to trigger cell sorting)
  • Conduct microfluidic experiments
  • Construct a prototype and characterize the performance
  • Have basic knowledge about other types of lab equipment
  • Describe the basic working principles of other lab equipment

Transversal skills

  • Collect data.
  • Make an oral presentation.
  • Plan and carry out activities in a way which makes optimal use of available time and other resources.
  • Set objectives and design an action plan to reach those objectives.
  • Communicate effectively with professionals from other disciplines.
  • Write a scientific or technical report.

Teaching methods

  • Teaching is done through interactive lectures, including case studies presented by the students
  • In parallel, the students go through a hands-on practical session every week ("learning by doing"), comprising the construction of a fully functional bioinstrument

Expected student activities

Prepare a blueprint for a droplet microfluidic workstation, based on a comprehensive protocol

Present a bioinstrument of your choice (to be approved), based on comprehensive literature search

Construct a functional bioinstrument, collect data, present results in oral and written form

 

Assessment methods

Presentation of a bioinstrument of your choice (to be approved; Group activity, 30%)

Construction of a fully functional microfluidic instrument, presentation of experimental results (Group activity; 30%), submission of a written report (Group activity, 20%)

Q & A sessions with individual students to initiate a discussion about the performed work (Individual activity, 20%)

Supervision

Office hours Yes
Assistants Yes
Forum Yes

Resources

Notes/Handbook

Protocols, slides and literature will be made available on Moodle

In the programs

  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Semester: Fall
  • Number of places: 18
  • Exam form: During the semester (winter session)
  • Subject examined: Basics in bioinstrumentation
  • Lecture: 1 Hour(s) per week x 14 weeks
  • Exercises: 1 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks
  • Labs: 2 Hour(s) per week x 14 weeks

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     

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