Advanced bioengineering methods laboratory

Summary

Advanced Bioengineering Methods Laboratories (ABML) offers laboratory practice and data analysis. These active sessions present a variety of techniques employed in the bioengineering field and matching a quantitative and technological based approach.

Content

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Keywords

Atomic force microscopy (AFM), Lab on the chip (LOC) , Brownian motion, Optical trapping , Surface Plasmon Resonance. bioanalytics, surface design, writing scientific papers

Learning Prerequisites

Required courses

Required background: Biophysics I, Biothermodynamics, Biomicroscopy I, + mandatory courses of M1

Expected student activities

Beyond the work requested during the supervised sessions (practice and analysis), the student will have to:

• Read the introduction of each topic before the corresponding practice, and summarize this information in his laboratory notebook.
• 
Review the data analysis tools needed for the analysis sessions and prepare the required calculations ahead of the corresponding analysis session.
• Fill the laboratory notebook progressively along the semester.
• Develop a research plan for the independent project
• Write the research paper

The workload varies widely with the capabilities of each student. However, we expect, for each of the 6 topics investigated, an approximate working time of

• 2 h : Preparation of the practical session

• 4 h : Practical session 

 

Resources

Bibliography

• Handouts given during the course.
• Intermolecular and Surface Forces, J. Israelachvili, Academic press

• Surface Plasmon resonance Based Sensors, J.Homola et al., Springer

• Surface Design: Applications in Bioscience and Nanotechnology, R. Forch, H. Schonherr, A.T. Jenkins, Wiley
• "Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements," Taylor, John R., 1997, University Science Books,
• Optical Trapping Review : K.C. Neuman & S.M. Block, "Optical trapping,"
Rev. Sci. Instrum. 75 (2003).
• Lab on a Chip Technology, Volume 1: Fabrication and Microfluidics, Keith E. Herold and Avraham Rasooly, Caister Academic Press, 2009
• Atomic Force Microscopy, Peter Eaton and Paul West, Oxford University Press 2010

Ressources en bibliothèque

• Intermolecular and Surface Forces / Israelachvili

• Surface Plasmon resonance Based Sensors / Homola

• Surface Design: Applications in Bioscience and Nanotechnology / Forch
• Introduction to Error Analysis / Taylor
• Optical Trapping Review / Neuman

• Lab on a Chip Technology / Herold
• Atomic force microscopy/ Peter Eaton ; Paul West (http://beast-epfl.hosted.exlibrisgroup.com/primo_library/libweb/action/search.do?cs=frb&ct=frb&frbg=&fctN=facet_frbrgroupid&fctV=104137913&doc=TN_ebi01_prod006075632&lastPag=&lastPagIndx=1&rfnGrp=frbr&dscnt=0&frbg=&scp.scps=scope%3A(EPFL_SFX)%2CPrimo2Primo_41EPFL&tab=default_tab&dstmp=1433946714337&srt=rank&ct=search&mode=Basic&&dum=true&tb=t&indx=1&vl(freeText0)=Paul%20West&vid=EPFL&fn=search#)

 

Ressources en bibliothèque

• Optical Trapping Review / Neuman
• Surface Plasmon resonance Based Sensors / Homola
• An introduction to Error Analysis / Taylor
• Lab on a Chip Technology / Herold
• Atomic force microscopy/ Peter Eaton ; Paul West
• Intermolecular and Surface Forces / Israelachvili
• Surface Design: Applications in Bioscience and Nanotechnology / Förch

Notes/Handbook

Can be downloaded from

http://lben.epfl.ch/Teaching

 

Moodle Link

• https://go.epfl.ch/BIOENG-444