BIOENG-444 / 4 credits

Teacher(s): Radenovic Aleksandra, Guiducci Carlotta, Fantner Georg

Language: English

Withdrawal: It is not allowed to withdraw from this subject after the registration deadline.

Remark: The course is held in an amended format giving more freedom to students to learn plan and perform research


Summary

Advanced Bioengineering Methods Laboratories (ABML) offers laboratory practice and data analysis. These active sessions present a variety of techniques employed in the bioengineering field and matching a quantitative and technological based approach.

Content

Keywords

Atomic force microscopy (AFM), Lab on the chip (LOC) , Brownian motion, Optical trapping , Surface Plasmon Resonance. bioanalytics, surface design, writing scientific papers

Learning Prerequisites

Required courses

Required background: Biophysics I, Biothermodynamics, Biomicroscopy I, + mandatory courses of M1

Expected student activities

Beyond the work requested during the supervised sessions (practice and analysis), the student will have to:

  • Read the introduction of each topic before the corresponding practice, and summarize this information in his laboratory notebook.
  • 
Review the data analysis tools needed for the analysis sessions and prepare the required calculations ahead of the corresponding analysis session.
  • Fill the laboratory notebook progressively along the semester.
  • Develop a research plan for the independent project
  • Write the research paper

The workload varies widely with the capabilities of each student. However, we expect, for each of the 6 topics investigated, an approximate working time of

  • 2 h : Preparation of the practical session

  • 4 h : Practical session 

 

Resources

Bibliography

  • Handouts given during the course.
  • Intermolecular and Surface Forces, J. Israelachvili, Academic press

  • Surface Plasmon resonance Based Sensors, J.Homola et al., Springer

  • Surface Design: Applications in Bioscience and Nanotechnology, R. Forch, H. Schonherr, A.T. Jenkins, Wiley
  • "Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements," Taylor, John R., 1997, University Science Books,
  • Optical Trapping Review : K.C. Neuman & S.M. Block, "Optical trapping,"
Rev. Sci. Instrum. 75 (2003).
  • Lab on a Chip Technology, Volume 1: Fabrication and Microfluidics, Keith E. Herold and Avraham Rasooly, Caister Academic Press, 2009
  • Atomic Force Microscopy, Peter Eaton and Paul West, Oxford University Press 2010

Ressources en bibliothèque

  • Intermolecular and Surface Forces / Israelachvili

  • Surface Plasmon resonance Based Sensors / Homola

  • Surface Design: Applications in Bioscience and Nanotechnology / Forch
  • Introduction to Error Analysis / Taylor
  • Optical Trapping Review / Neuman

  • Lab on a Chip Technology / Herold
  • Atomic force microscopy/ Peter Eaton ; Paul West (http://beast-epfl.hosted.exlibrisgroup.com/primo_library/libweb/action/search.do?cs=frb&ct=frb&frbg=&fctN=facet_frbrgroupid&fctV=104137913&doc=TN_ebi01_prod006075632&lastPag=&lastPagIndx=1&rfnGrp=frbr&dscnt=0&frbg=&scp.scps=scope%3A(EPFL_SFX)%2CPrimo2Primo_41EPFL&tab=default_tab&dstmp=1433946714337&srt=rank&ct=search&mode=Basic&&dum=true&tb=t&indx=1&vl(freeText0)=Paul%20West&vid=EPFL&fn=search#)

 

Notes/Handbook

Can be downloaded from

http://lben.epfl.ch/Teaching

 

In the programs

  • Semester: Spring
  • Number of places: 18
  • Exam form: During the semester (summer session)
  • Subject examined: Advanced bioengineering methods laboratory
  • Practical work: 4 Hour(s) per week x 14 weeks
  • Semester: Spring
  • Number of places: 18
  • Exam form: During the semester (summer session)
  • Subject examined: Advanced bioengineering methods laboratory
  • Practical work: 4 Hour(s) per week x 14 weeks

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22