Fiches de cours 2017-2018

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Protein mass spectrometry and proteomics

CH-419

Enseignant(s) :

Dayon Loïc

Langue:

English

Summary

In systems biology, proteomics represents an essential pillar. The understanding of protein function and regulation provides key information to decipher the complexity of living systems. Proteomic technology now enables deep quantitative proteome mapping via mass spectrometry.

Content

1. Introduction

Introduction to protein analysis and proteomics; Reminders in mass spectrometry; Why proteomics and mass spectrometry?; Ionization sources, analyzers, and detectors used in proteomics

2. Proteomic strategy and workflows

Bottom-up versus top-down strategies; Sample preparation

3. Separations techniques in proteomics

Gel electrophoresis; Isoelectric focusing; Liquid chromatography (RP, IEX)

4. Quantitative proteomic workflows

Label-free methods; Labelling-based techniques; Other quantitative techniques

5. Proteomic bioinformatics

Databases; Identification of protein; Quantification of proteins; Bioinformatics tools; Practical examples

6. Applications to biology and clinical research

What strategy?; Experimental design; Biomarker discovery; Industrialized and population proteomics; Targeted mass spectrometry-based approaches; Other biological applications of mass spectrometry; Lab visit

Keywords

Mass Spectrometry; Liquid Chromatography; Electrophoresis; Proteins; Peptides; Systems Biology; Bioinformatics; Human Samples; Clinics; Isotopic Labelling; Quantification

Learning Prerequisites

Recommended courses

CH-210 Biochimie I

CH-301 Méthodes de séparation analytiques

CH-405 Methodology in instrumental chromatography

CH-403 Mass spectrometry

Important concepts to start the course

Mass spectrometry; Biochemistry

Learning Outcomes

By the end of the course, the student must be able to:

Teaching methods

Ex-cathedra

Assessment methods

Multiple Choice Question (MCQ) exam

Presentation during the course

Supervision

Office hours No
Assistants No
Forum No

Resources

Ressources en bibliothèque
Références suggérées par la bibliothèque
Websites

Dans les plans d'études

Semaine de référence

 LuMaMeJeVe
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
En construction
 
      Cours
      Exercice, TP
      Projet, autre

légende

  • Semestre d'automne
  • Session d'hiver
  • Semestre de printemps
  • Session d'été
  • Cours en français
  • Cours en anglais
  • Cours en allemand