Fiches de cours 2017-2018

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Lab methods : flow cytometry

BIOENG-514

Enseignant(s) :

Garcia Miguel

Langue:

English

Withdrawal

It is not allowed to withdraw from this subject after the registration deadline.

Remarque

Inscriptions sur dossier auprès du responsable du cours; présence aux cours obligatoire

Summary

This module cover the fundamentals of Flow Cytometry from theory to practical applications, including practice of acquisition and cell sorting experiments. Students work in small groups for hands-on time, putting theory into practice in the laboratory.

Content

This module will cover a wide range of topics on Flow Cytometry and Cell Sorting. These lectures will start from the basics and move right through to the complicated aspects of flow cytometry for analysis and cell sorting. This module will be separated into three theoretical lectures, one demonstration, a "practical part" and articles discussion.

The followings topics will be introduced :

 

First principles of flow cytometry

 

Principles of Multicolour flow cytometry

 

Principles on cell sorting

Keywords

Flow Cytometry, fluorescence, multicolour, panel design, compensation, cell sorting

Learning Prerequisites

Required courses

First year of master in Life Sciences & Technology or Bioengineering program.

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

Ex-cathedra lectures to introduce the theory followed by desmonstration and "hands-on" on practical sessions in the Laboratory including sample acquisition, analysis and data interpretation. Discussion on selected papers representative of the technique used in Flow Cytometry.

Registration forms must be sent together with a cover letter clearly stating your interest in this technique. Enrolment will be validated by the teacher on a motivational basis.

This course will take place from October 24th to October 28th, 2016.

Assessment methods

Written exam (2 hours)

Supervision

Office hours Yes
Assistants Yes
Forum No
Others

Office Hours : 9:00 - 17:00

Assistants : Loïc Tauzin

Resources

Websites
Moodle Link

Dans les plans d'études

Semaine de référence

 LuMaMeJeVe
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
 
      Cours
      Exercice, TP
      Projet, autre

légende

  • Semestre d'automne
  • Session d'hiver
  • Semestre de printemps
  • Session d'été
  • Cours en français
  • Cours en anglais
  • Cours en allemand