Coursebooks 2017-2018

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Practical - Blokesch Lab

BIO-666

Lecturer(s) :

Blokesch Melanie

Language:

English

Frequency

Every year

Remarque

3-day Block course, every year in January. To register, contact EDMS Administration

Summary

How to look at tiny things: visualizing protein localization in bacteria using epifluorescence microscopy.

Content

Theory:
Basics on protein localization in bacteria.


Practical part:
Preparing bacteria for microscopy.
Staining methods to visualize bacteria.
Epifluorescence microscopy.
Comparison of protein localization depending on protein levels (varying artifical induction).
Basic image analysis (MicrobeTracker).
Independent analysis of an unknown bacterium for cell shape and potential localized fluorescently labeled proteins.

Note

Note that while the course is open to all first year EPFL doctoral students, priority will be given to EDMS students, given that they are mandated to take three of EDMS practical modules. Doctoral students from the Blokesch laboratory cannot take this course. Minimum 2 students, max. 4 students.

Resources

Websites

In the programs

Reference week

 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German