Coursebooks 2016-2017

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Nanobiotechnology and biophysics

CH-413

Lecturer(s) :

Fierz Beat

Language:

English

Summary

This course concerns modern bioanalytical techniques to investigate biomolecules both in vitro and in vivo, including recent methods to image, track and manipulate single molecules. We cover the basic principles of the respective methods and discuss examples from the current scientific literature.

Content

Techniques to monitor the function of single biomolecules and complexes

  1. single molecule fluorescence spectroscopy (FRET, confocal and total internal reflection fluorescence microscopy
  2. - Force spectroscopy to monitor function of single proteins and cells
  3. - Microscopy beyond the diffraction limit: Super-resolution microscopy

 

Surface sensors to elucidate and quantify molecular interactions:

- Immobilizing biopolymers on surfaces

- Optical & electrical detection techniques

 

Development and application of microfluidic and nanofluidic sensor devices:

- Miniaturisation of analytical techniques: lab on a chip

- Chemical and biochemical sensors

- Next generation DNA sequencing approaches

 

Engineered biomolecules to manipulate cells or as drug delivery vehicles

- Nano-containers for drug delivery vectors

- DNA based self-assembly and nanofabrication of complex structures

Keywords

Nanobiotechnology, biophysics, sensors, single-molecule, fluroescence, FRET, drug delivery, DNA origami, lab-on-a-chip, super-resolution microscopy, force spectroscopy

Learning Prerequisites

Required courses

Biochemistry I and II

Molecular and Cellular Biophysics I and II

Important concepts to start the course

Biomolecular absorption and fluroescence

General biochemistry

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

Ex cathedra, presentations by students and paper discussions

Expected student activities

Discussion of recent literature in the form of presentations

Assessment methods

Written exam

Supervision

Assistants Yes
Others Moodle

Resources

Bibliography

Nanobiotechnology, Niemeyer & Merkin (Wiley 2004)

Introduction to bioMEMS, Folch (CRC press, 2012)

Ressources en bibliothèque
Notes/Handbook

Handouts and literature references distributed in class

Websites

In the programs

Reference week

 MoTuWeThFr
8-9  CHB330  
9-10    
10-11  CHB330  
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German