Coursebooks 2016-2017

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Molecular and cellular biophysic I

CH-311

Lecturer(s) :

Fierz Beat

Language:

English

Summary

This course covers the basic biophysical principles governing the thermodynamic and kinetic properties of biomacromolecules involved in chemical processes of life. The course is held in English.

Content

  1. The conformation of biological macromolecules and membranes
    1. Forces in biomolecules
    2. Protein primary and secondary structure
    3. Tertiary structure of proteins
    4. DNA structure
    5. Conformations of unstructured polymers in solution (Gaussian chain models, freely-jointed chain, wormlike chain)
  2. Spectroscopy of Biomolecules
    1. Biomolecular absorption spectroscopy (UV absorption, circular dichroism)
    2. Biomolecular fluorescence
    3. X-ray crystallography of proteins
  3. Conformational equilibria and dynamics of polypeptides and proteins
    1. Thermodynamics of protein folding (folding equilibria, calorimetry of protein folding transitions)
    2. Kinetics of protein folding (folding pathways, intermediates)
    3. Conformational transitions in proteins (native state fluctuations, allostery, structural rearrangements in enzyme catalysis)
    4. Thermodynamics and kinetics of alpha-helix -€“ coil transitions
  4. Transport phenomena and stochastic processes in biology
    1. Fluctuations in biology
    2. Macromolecular diffusion
  5. Thermodynamics and kinetics of ligand-receptor interactions
    1. Equilibrium binding reactions
    2. Binding inhibition
    3. Kinetics of ligand binding

Keywords

biophysics, biophysical chemistry, protein, nucleic acid, structure, thermodynamics, kinetics, protein folding, spectroscopy, fluorescence, absorption, helix-coil, fluctuations, receptor, ligand

Learning Prerequisites

Required courses

Biochemistry I

Chemical thermodynamics

Important concepts to start the course

General chemical and biochemical concepts

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

ex cathedra

Expected student activities

Attendance of the lectures

Study of indicated materials

Assessment methods

Written exam

Resources

Bibliography

"Biophysical Chemistry", Cantor and Schimmel, Vols 1-3 (Freeman, New York 1980)

"Molecular and Cellular Biophysics", Meyer B. Jackson (Cambridge University Press, 2006)

Ressources en bibliothèque

Prerequisite for

Molecular biophysics II

Experimental biological & biophysical chemistry

Nanobiotechnology and Biophysics

Cellular Signaling

In the programs

    • Semester
       Fall
    • Exam form
       Written
    • Credits
      2
    • Subject examined
      Molecular and cellular biophysic I
    • Lecture
      2 Hour(s) per week x 14 weeks
  • Passerelle HES - CGC, 2016-2017, Autumn semester
    • Semester
       Fall
    • Exam form
       Written
    • Credits
      2
    • Subject examined
      Molecular and cellular biophysic I
    • Lecture
      2 Hour(s) per week x 14 weeks

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
Under construction
 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German