Coursebooks 2017-2018

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Mechanisms of cell motility

BIO-714

Lecturer(s) :

Verkhovskiy Alexander

Language:

English

Frequency

Every 2 years

Remarque

Next time: Spring 2019

Summary

Mechanisms of cell motility

Content

1. Overview of different types of cell motility. Mechanisms of bacterial motility and chemotaxis.
2. Eucaryotic cell motility: flagellated and ciliated cells, crawling cell motility.
3. Motile machinery: types of the cytoskeletal structures, principles of assembly of cytoskeletal filaments (treadmilling, dynamic instability).
4. Motile machinery: accessory proteins, regulation of assembly and supramolecular organization of the cytoskeleton.
5. Methods to study cytoskeletal dynamics: live digital fluorescence microscopy, photoactivation, photobleaching, fluorescence speckle microscopy, and others.
6. Mechanisms of actin assembly in protrusion at the leading edge of the cell.
7. Biophysics of protrusion, forces and modeling.
8. Cell-substrate attachment: molecular composition and dynamics of adhesion sites.
9. Introduction to motor proteins, active cycle, steps and forces.
10. Microtubule-dependent motors, role in intracellular transport and mitosis.
11. Myosin superfamily of motor proteins, non-conventional myosins in intracellular transport and hearing.
12. Myosin II, coordination of protrusion, attachment and contraction in the cell translocation.
13. Signaling to motility and the origins of cell polarity (directional sensing in chemotaxis, PH-domain proteins, small GTPases, calcium).
14. Interaction between actin and microtubules in cell polarization and motility (budding yeast model, animal cell mitosis and cytokinesis, cell migration).

 

Note

Learning outcomes

The course will be given in 14 weekly sessions, each either a 2h lecture or 1.5h lecture followed by 30 min presentation by one of the attendees and a guided group discussion. Presenters will discuss original papers related to this day's lecture topic.

 

This course requires a minimum of 4 participants and is limited to 15 participants.

Keywords

cell migration, cytoskeleton, actin, myosin, microtubules

Learning Prerequisites

Recommended courses

basic biology

Assessment methods

Oral

In the programs

Reference week

 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German