Coursebooks 2017-2018

• Directory
• EPFL websites
• Place
• Courses
• Publications
• News
• EPFL units

Search

 

Mass spectrometry, principles and applications

CH-728

Lecturer(s) :

Menin Laure
Michalet Sophie
Moniatte Marc
Ortiz Trujillo Daniel
Patiny Luc
Paz Montoya Jonathan
Rochat Bertrand
Sepulveda Francisco

Language:

English

Frequency

Every year

Remarque

Next time: Block January 2018 (1 week)+ September 2018(1 week)

Summary

The goal is to provide students with a complete overview of the principles and key applications of modern mass spectrometry and meet the current practical demand of EPFL researchers to improve structural analsis of molecules. Numerous instrumental aspects of mass spectrometry are described.

Content

The course program includes:

• Mass spectrometry basics (ionization techniques, mass analyszers and detectors) and applications
• Tandem mass spectrometry and hyphenation with separation (UPLC and GC) systems
• Fragmentation mechanisms/techniques and structural analsis for identification of compounds
• Fourier-Transform Mass spectrometry (Orbitrap and ICR mass analyzers)
• Quantification (absolute and relative) methods with low resolution (triple quadrupole) and high-resolution (QTOF and Orbitrap) instruments
• Analysis of biomolecules such as bottom-up and top-down MS for peptide/protein analysis, analysis of binding sites of metallated-ligands on peptides'
• Open-source tools for Advanced analysis of mass spectra
• Proteomics, lipidomics and metabolomics fields

The course includes practical work in mass spectrometry that will be given mostly in the Mass Spectrometry Service Facility of ISIC (SSMI, SB, EPFL) and in the Proteomic Core Facility (PCF, SV, EPFL).

Note

January and September 2017 (block) Full

Keywords

mass spectrometry, tandem mass spectrometry, High-resolution mass spectrometry (HRMS), liquid chromatography, Gas chromatography, quantification, proteomics, lipidomics, metabolomics, proteomics

Assessment methods

Oral