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Coursebooks 2017-2018
Mass spectrometry, principles and applications
CH-728
Lecturer(s) :
Boyarkine OlegGasilova Natalia
Menin Laure
Ortiz Trujillo Daniel
Patiny Luc
Sepulveda Francisco
Language:
English
Frequency
Every yearRemarque
Next time: March+September 2018Summary
The goal is to provide students with a complete overview of the principles and key applications of modern mass spectrometry and meet the current practical demand of EPFL researchers to improve structural analsis of molecules. Numerous instrumental aspects of mass spectrometry are described.Content
The course program includes:
Week 1
1. Lectures
1.1 General introduction to MS: Definitions/Instrumentation
1.2 MS/MS:fragmentation methods and mechanisms; Ion Mobility MS
1.3 LC-MS and other hyphenated techniques
1.4 ICP-MS
1.5 Summary of all concepts and fundamental MS aspects seen during the week.
2. Exercises:
Ms.cheminfo org tools for advanced MS
3. Pratical work:
3.1 Fragmentation of small molecules (sugars, small peptides) using QTOF and FT-Orbitrap- MS, fragmentation by EI; interpretation of mass spectra using different tools and softwares.
3.2 Individual work for each doctorant topics.
Week 2
1. Lectures
1.1 High Resolution Mass spectrometry
1.2 Photo-dissociation spectroscopy mass spectrometry
1.3 Peptidomics/Proteomics: top-down and bottom-up approaches
1.4 MALDI-TOF: principles and applications
1.5 Ion Mobility MS
2. Exercises
Exercices on all topics covered by the MS course
3. Final exam
15'presentation of each PhD student of its individual Practical Work
The course includes practical work in mass spectrometry that will be given in the Mass Spectrometry Service Facility of ISIC (SSMI, SB, EPFL).
Note
1 week early March and 2nd week early September 2018 (block)
Keywords
mass spectrometry, tandem mass spectrometry, High-resolution mass spectrometry (HRMS), liquid chromatography, Gas chromatography, quantification, proteomics, lipidomics, metabolomics, proteomics
Assessment methods
Oral
In the programs
- Semester
- Exam form
Oral - Credits
3 - Subject examined
Mass spectrometry, principles and applications - Lecture
23 Hour(s) - Exercises
8 Hour(s) - Practical work
27 Hour(s)
- Semester
Reference week
Lecture
Exercise, TP
Project, other
legend
- Autumn semester
- Winter sessions
- Spring semester
- Summer sessions
- Lecture in French
- Lecture in English
- Lecture in German