Coursebooks 2017-2018

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Lab methods : flow cytometry

BIOENG-514

Lecturer(s) :

Garcia Miguel

Language:

English

Withdrawal

It is not allowed to withdraw from this subject after the registration deadline.

Remarque

Inscriptions sur dossier auprès du responsable du cours; présence aux cours obligatoire

Summary

This module covers the fundamentals of Flow Cytometry, both practical and theoretical. Students will work in small groups, acquiring samples and preparing cell sorting experiments, giving them hands-on time and allowing them to put the theory into practice inside a laboratory environment.

Content

This module will cover a wide range of topics on Flow Cytometry and Cell Sorting. These lectures will start from the basics and move into the complicated aspects of flow cytometry for analysis and cell sorting. This module will be divided into three theoretical lectures, one hands-one session, a "practical part" and articles discussion.

The followings topics will be introduced :

 

First principles of flow cytometry

 

Principles of Multicolour flow cytometry

 

Principles on cell sorting

Keywords

Flow Cytometry, fluorescence, multicolour, panel design, compensation, cell sorting

Learning Prerequisites

Required courses

First year of master in Life Sciences & Technology or Bioengineering program.

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

Ex-cathedra lectures to introduce the theory followed by desmonstration and "hands-on" on practical sessions in the Laboratory including sample acquisition, analysis and data interpretation. Discussion on selected papers representative of the technique used in Flow Cytometry.

Registration forms must be sent together with a cover letter clearly stating your interest in this technique. Enrolment will be validated by the teacher on a motivational basis.

This course will take place Fall semester 2017-2018.

Assessment methods

Written exam (2 hours)

Supervision

Office hours Yes
Assistants Yes
Forum No
Others

Office Hours : 9:00 - 17:00

Assistants : André Mozes & Christoph Scwärzler

 

Resources

Websites
Moodle Link

In the programs

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German