Coursebooks 2017-2018

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Lab methods : bioactive compounds screening

BIOENG-512

Lecturer(s) :

Chambon Marc
Turcatti Gerardo

Language:

English

Withdrawal

It is not allowed to withdraw from this subject after the registration deadline.

Remarque

Schedule : November 13 to 17, 2017 (from 9AM to 5PM) - présence aux cours obligatoire

Summary

Introduction to the key principles and concepts underlying the screening activity to identify and to characterize bioactive compounds (chemicals, compounds, siRNAs and natural products) acting on a given biological target or a signalling pathway.

Content

Keywords

Screening / Drug discovery / Assay development /Assay validation/ z' factor / siRNA / Transfection / Cell viability / Enzymatic activity / Inhibitor / Dose response

 

Learning Prerequisites

Required courses

Chemical Biology, Biochemistry, Cell Biology

Recommended courses

Bio-494 scientific project design in drug discovery

Important concepts to start the course

Basis of cell biology (cell viability, cytotoxicity, RNAinterference, transfection)

Basis of biochemistry (enzymology, inhibition)

Basic knowledge in chemistry (physical, analytical and organic chemistry)

Basic knowledge of statistics applied to biology

Learning Outcomes

By the end of the course, the student must be able to:

Transversal skills

Teaching methods

Ex-cathedra courses:Theoretical introduction and case studies presentations

Practical work: Performing experiments including results analyses

Interactive discussion of generated data and biochemical/biological relevance

Training and utilisation of instrumental devices under expert supervision (readers, liquid handlers, automated microscope)

Attend robotic workstations demos during the visit of the screening platform labs.

Expected student activities

Attendance to the whole course

Read and understand experimental protocols

Carry out experiments in the lab (by group of 2)

Analyze daily results (basic statistic with computer), including critical evaluation

Share data and involvment in general discussion

Read some general papers of interest selected and related to the course

 

Assessment methods

Written examination at the end of the course

In the programs

Reference week

 MoTuWeThFr
8-9     
9-10     
10-11     
11-12     
12-13     
13-14     
14-15     
15-16     
16-17     
17-18     
18-19     
19-20     
20-21     
21-22     
 
      Lecture
      Exercise, TP
      Project, other

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  • Autumn semester
  • Winter sessions
  • Spring semester
  • Summer sessions
  • Lecture in French
  • Lecture in English
  • Lecture in German